Coding
ninaB

Part:BBa_K343001:Design

Designed by: Christian Kurtzhals   Group: iGEM10_SDU-Denmark   (2010-07-02)

B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
    Illegal BamHI site found at 420
    Illegal BamHI site found at 1677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1281
    Illegal BsaI site found at 1729


Design Notes

The sequence is isolated from a cDNA, starting from the first Met residue after the second to last stop codon of the correct reading frame, and ending at final stop. It matches the number of residues in the desired protein.

Source

Sequence taken from cDNA from Ruben GM, et. al.

References

[http://www.sciencemag.org/cgi/content/abstract/287/5461/2222A] Drosophila Complementary DNA Resource Rubin et al. Science 24 March 2000: 2222 DOI: 10.1126/science.287.5461.2222

[http://flybase.org/reports/FBcl0129473.html]FlyBase.org cDNA reference